Part:BBa_K4193026:Design
QS system
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Design Notes
Cytokinin isopentenyladenine (IP), as quorum sensing molecule, is the product of ATP catalyzed by AtlPT4. When the signal molecule IP reaches a certain concentration, it activates the SSRE promoter and increases the expression of production gene controlled by SSRE promoter in our experiment, to better characterize SSRE promoter and quorum sensing circuit, we put eGFP gene at the downstream of SSRE promoter.
We successfully introduced cytokinin-mediated quorum sensing circuit into the genome of Aureobasidium melanogenum P16. To characterize this circuit and SSRE promoter, we added different concentration of IP and introduced eGFP at the downstream of SSRE promoter. We measured the fluorescence to figure out the dynamic regulation range.
Source
synthesize from company
References
Yang, X., Liu, J., Zhang, J., Shen, Y., Qi, Q., Bao, X. and Hou, J. (2021). Quorum sensing-mediated protein degradation for dynamic metabolic pathway control in Saccharomyces cerevisiae. Metabolic Engineering, 64, pp.85–94. doi:10.1016/j.ymben.2021.01.010.